Application | Comment | Organism |
---|---|---|
industry | possible use of the enzyme in detergent industry and peptide synthesis due to its compatibility with several detergents, oxidants and organic solvents | Aspergillus flavus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | about 10% inhibition at 5 mM | Aspergillus flavus | |
Cu2+ | over 90% inhibition at 5 mM | Aspergillus flavus | |
Hg2+ | complete inhibition at 5 mM | Aspergillus flavus | |
Mn2+ | about 30% inhibition at 5 mM | Aspergillus flavus | |
additional information | poor inhibition by Ca2+ at 5 mM | Aspergillus flavus | |
Ni2+ | about 20% inhibition at 5 mM | Aspergillus flavus | |
Zn2+ | about 20% inhibition at 5 mM | Aspergillus flavus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Aspergillus flavus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | activates 1.74fold at 5 mM | Aspergillus flavus | |
K+ | activates about 10% at 5 mM | Aspergillus flavus | |
additional information | no effect on enzyme activity by 5 mM Pb2+. Effect of metal salts on enzyme production, overview | Aspergillus flavus | |
Na+ | activates about 10% at 5 mM | Aspergillus flavus |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 97.1% remaining activity after 1 h at 30°C in 25% organic solvent | Aspergillus flavus |
benzene | 89.2% remaining activity after 1 h at 30°C in 25% organic solvent | Aspergillus flavus |
Butanol | 96.2% remaining activity after 1 h at 30°C in 25% organic solvent | Aspergillus flavus |
chloroform | 105.3% activity after 1 h at 30°C in 25% organic solvent | Aspergillus flavus |
diethyl ether | 89.2% remaining activity after 1 h at 30°C in 25% organic solvent | Aspergillus flavus |
Ethanol | 83.2% remaining activity after 1 h at 30°C in 25% organic solvent | Aspergillus flavus |
hexane | 94.3% remaining activity after 1 h at 30°C in 25% organic solvent | Aspergillus flavus |
Methanol | 81.7% remaining activity after 1 h at 30°C in 25% organic solvent | Aspergillus flavus |
additional information | extremely organic solvent stable serine protease. The enzyme retains 80% of its original activity in the presence of non ionic and ionic surfactants after 1 h of incubation at 30°C, overview | Aspergillus flavus |
toluene | 91.7% remaining activity after 1 h at 30°C in 25% organic solvent | Aspergillus flavus |
xylene | 83.6% remaining activity after 1 h at 30°C in 25% organic solvent | Aspergillus flavus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus flavus | - |
- |
- |
Aspergillus flavus MTCC 9952 | - |
- |
- |
Oxidation Stability | Organism |
---|---|
oxidant stable serine protease. The enzyme retains 100% of its original activity with 10% H2O2 after 1 h of incubation at 30°C | Aspergillus flavus |
Purification (Comment) | Organism |
---|---|
native enzyme partially by acetone precipitation | Aspergillus flavus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the organism is grown under solid state fermentation. Maximum protease yield is obtained when the strain is grown under wheat bran and corn cob mixture, ratio 1:1, incubated for 48 h at pH 9.0 and temperature 37°C with 50% of initial moisture content, evaluation overview | Aspergillus flavus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | artificial substrate | Aspergillus flavus | ? | - |
? | |
casein + H2O | artificial substrate | Aspergillus flavus MTCC 9952 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Aspergillus flavus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | 100% activity at 40°C while 92% residual activity at 45°C, 74% at 50°C, and 41% 55°C, inactive above 55°C, temperature profile, overview | Aspergillus flavus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermal stability profile, overview | Aspergillus flavus |
30 | 40 | partially purified enzyme, stable at, retention of 96% activity after 1 h of incubation at 40°C | Aspergillus flavus |
45 | - |
84% residual activity after 1 h of incubation at 45°C | Aspergillus flavus |
55 | - |
53% residual activity after 1 h of incubation at 55°C | Aspergillus flavus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 12 | - |
Aspergillus flavus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 12 | partially purified enzyme, stable at | Aspergillus flavus |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme retains 80% of its original activity in the presence of non ionic and ionic surfactants after 1 h of incubation at 30°C, overview | Aspergillus flavus |